CEL-SCI Study Shows CEL-2000 Vaccine Blocks Progression of Rheumatoid Arthritis
22 Février 2010 - 3:45PM
PR Newswire (US)
Statistically Significant Findings Published in Prestigious Journal
of International Immunopharmacology VIENNA, Va., Feb. 22
/PRNewswire-FirstCall/ -- CEL-SCI Corporation (NYSE AMEX: CVM) and
their scientific collaborators announced today that the Company's
CEL-2000 vaccine demonstrated that it is able to block the
progression of rheumatoid arthritis (RA) in a mouse model. The
results were published in the scientific peer-reviewed Journal of
International Immunopharmacology (online edition) in an article
titled "CEL-2000: A Therapeutic Vaccine for Rheumatoid Arthritis
Arrests Disease Development and Alters Serum Cytokine / Chemokine
Patterns in the Bovine Collagen Type II Induced Arthritis in the
DBA Mouse Model" with lead author Dr. Daniel Zimmerman. The study
was co-authored by scientists from CEL-SCI, Washington Biotech,
Northeastern Ohio Universities Colleges of Medicine and Pharmacy
(NEOUCOMP) and Boulder BioPath. CEL-2000, administered after
disease (RA) symptoms had started, prevented, in a statistically
significant manner, the further development of arthritic
conditions, including joint swelling and deformation, bone and
cartilage changes and was accompanied by serum cytokine alterations
over the CEL-2000 treatment period with comparable or better
activity than the well accepted etanercept (Enbrel®) therapy. The
mode of action is very consistent with the findings of induction of
IL-12 followed by interferon gamma and an inhibition of TNF-alpha
and IL-17 production. TNF-alpha and IL-17 are both key cytokines
for induction of the pathology seen in rheumatoid arthritis and
TNF-alpha is the target of many current RA therapies such as
Enbrel, Remicaid, and Humaria. The protection effect mediated by
CEL-2000 treatment against RA was also demonstrated histologically
with significant reductions in: 1) inflammation, 2) cartilage
destruction, 3) bone resorption, and 4) pannus membrane formation
in the synovial space compared to untreated controls. Geert
Kersten, Chief Executive Officer of CEL-SCI said, "These
experimental results were achieved through a reduction of the
inflammatory response that is known to attack the patients' joints.
The mode of action of CEL-2000 in RA appears to be similar to our
new investigational therapy for H1N1 hospitalized patients, as it
attempts to avoid the excess TNF-alpha and other pro-inflammatory
cytokines. We feel that this new data is encouraging both for this
rheumatoid arthritis vaccine as well as in support of our H1N1
treatment currently under development." In these studies, mice were
injected with collagen to induce the autoimmune (RA) disease.
Therapy with Enbrel or CEL-2000 was initiated after disease (RA)
symptoms have been established and treatment continued for 28 days
after the initiation of a significant, uniform, and measurable
level of arthritic disease in groups of mice. CEL-2000 was
administered only twice, however Enbrel had to be administered
every other day for the 28 day study period (as indicated for
Enbrel use). The extent of disease, as measured by deformation of
foot joints (Arthritic Index (AI) score), of untreated animals and
any improvements resulting from CEL-2000 and Enbrel treated animal
were then compared. In another study, CEL-2000 was administered 5
times over a 70 day period and the animals were monitored for a
total study period of 90 days. In each case, CEL-2000 treatment
proved effective in blocking progression of disease (RA) with
statistically significant reduction in AI score compared to
controls. The CEL-2000 treatment was deemed safe and well tolerated
without any reported adverse effects related to treatment. The
CEL-2000 treatment appeared to change the course of the immune
response in the diseased (RA) animals, limiting the development of
the destructive action of Th17 and tumor necrosis factor alpha
(TNF-alpha). Analysis of serum levels of 21 cytokines/chemokines
after 10 days of CEL-2000 treatment indicated reductions in the
characteristic cytokine markers of rheumatoid arthritis, TNF-alpha
and IL-17, as well as IL-6, and MCP-1. A number of cytokine changes
were also seen with Enbrel treatment, but to a lesser degree than
that seen with CEL-2000 treatment. CEL-2000 may also offer a number
of potential advantages over existing rheumatoid arthritis
treatments, such as Enbrel. Data collected in the animal studies
conducted with CEL-2000 demonstrated that CEL-2000 is an effective
treatment against rheumatoid arthritis even with administration of
many fewer treatments than for example Enbrel. CEL-2000 is also
potentially a more disease-type specific therapy, should be
significantly less expensive to manufacture, and finally, CEL-2000
could also be useful for patients who are not able to take or who
may be unresponsive to other existing anti-arthritis therapies.
This research featured the multidisciplinary team of collaborators
bringing to the project expertise of several different animal
models of arthritis (Washington Biotech), long time association
with modern molecular therapies and evaluation for RA (Bolder
Biopath), experience with other LEAPS immunogens, and experience
and expertise studying cytokines and with mechanistic studies of
the LEAPS technology (NEOUCOMP), and peptide technologies (21st
Century Biochemicals) to complement the expertise of the CEL-SCI
researchers. Rheumatoid arthritis treatments comprise an
approximately $13 billion market. Enbrel, a leading rheumatoid
arthritis treatment sold by Amgen and Wyeth, reported US sales in
2007 of about $3.2 billion. Enbrel is a soluble recombinant protein
of a human TNF-alpha receptor linked to human IgG Fc. In some
cases, human or humanized monoclonal antibodies specific against
TNF-alpha have also been used for therapy in rheumatoid arthritis.
These therapies remove or inactivate TNF-alpha, a natural human
cytokine required in many immune functions for normal defenses.
CEL-SCI's rheumatoid arthritis vaccine CEL-2000 was discovered as
part of work with the Company's ongoing research and development
activities with its L.E.A.P.S.(TM) (Ligand Epitope Antigen
Presentation System) technology. L.E.A.P.S. is a novel T-cell
modulation platform technology that enables CEL-SCI to design and
synthesize proprietary immunogens. Any disease for which an
antigenic sequence has been identified, such as infectious,
parasitic, malignant or autoimmune diseases and allergies, are
potential therapeutic or preventive sites for the application of
L.E.A.P.S. technology. The concept behind the L.E.A.P.S. technology
is to directly mimic cell-cell interactions and activate immune
cells with synthetic peptides. The L.E.A.P.S. constructs containing
the antigenic disease epitope linked to a immune-cell binding
ligand (ICBL) can be manufactured by peptide synthesis or by
covalently linking the two peptides. Depending upon the type of
L.E.A.P.S. construct and ICBL used, CEL-SCI is able to direct the
outcome of the immune response towards the development of T-cell
function with primarily effector T-cell functions (T Lymphocyte;
helper/effector T lymphocyte, type 1 or 2 [Th1 or Th2], cytotoxic
[Tc] or suppressor [Ts]). Therefore, it would appear that the
L.E.A.P.S. construct represents a chimeric peptide with
bi-functional behavior. Additional details including the full
publication with color photomicrographs of tissue sections, tables
and figures are available at
http://dx.doi.org/10.1016/j.intimp.2009.12.016. About CEL-SCI
Corporation CEL-SCI Corporation is developing products that empower
immune defenses. Its lead product Multikine is being readied for a
global Phase III trial in advanced primary head and neck cancer.
CEL-SCI is also developing an immunotherapy (LEAPS-H1N1-DC) to
treat H1N1 hospitalized patients and a vaccine (CEL-2000) for
Rheumatoid Arthritis using its L.E.A.P.S. technology platform. The
LEAPS-H1N1-DC treatment involves non-changing regions of H1N1
Pandemic Flu, Avian Flu (H5N1), and the Spanish Flu as CEL-SCI
scientists are very concerned about the creation of a new more
virulent hybrid virus through the combination of H1N1 and Avian
Flu, or maybe Spanish Flu. This investigational treatment is
currently being tested in a clinical study at Johns Hopkins
University. The Company has operations in Vienna, Virginia, and
in/near Baltimore, Maryland. For more information, please visit
http://www.cel-sci.com/. When used in this report, the words
"intends," "believes," "anticipated" and "expects" and similar
expressions are intended to identify forward-looking statements.
Such statements are subject to risks and uncertainties which could
cause actual results to differ materially from those projected.
Factors that could cause or contribute to such differences include,
lack of regulatory clearance to proceed with clinical trials, an
inability to duplicate the clinical results demonstrated in
clinical studies that have been completed or that are initiated in
the future, timely development of any potential products that can
be shown to be safe and effective, unwillingness of regulatory
authorities to engage in further regulatory dialogue, receiving
necessary regulatory approvals, difficulties in manufacturing any
of the Company's potential products, inability to raise the
necessary capital, and the risk factors set forth from time to time
in CEL-SCI Corporation's SEC filings, including but not limited to
its report on Form 10- K for the year ended September 30, 2009. The
Company undertakes no obligation to publicly release the result of
any revision to these forward-looking statements which may be made
to reflect the events or circumstances after the date hereof or to
reflect the occurrence of unanticipated events. DATASOURCE: CEL-SCI
Corporation CONTACT: Gavin de Windt of CEL-SCI Corporation,
+1-703-506-9460 Web Site: http://www.cel-sci.com/
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